Difference between revisions of "Part:BBa K1884011"
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We provided Paromomycin resistance under the RBCS2 promoter(BBa_K1884010) | We provided Paromomycin resistance under the RBCS2 promoter(BBa_K1884010) | ||
| − | === | + | ===Biology=== |
RBCS2 promoter | RBCS2 promoter | ||
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RBCS2 terminator is Chlamydomonas reinhardtii RuBisCO small subunit 2 terminator which obtained from the pOpt_mVenus_Hyg expression plasmid provided by Chlamydomonas Resource Center. | RBCS2 terminator is Chlamydomonas reinhardtii RuBisCO small subunit 2 terminator which obtained from the pOpt_mVenus_Hyg expression plasmid provided by Chlamydomonas Resource Center. | ||
| − | For testing the efficiency of paromoycin, we formulated six gradient concentrations of paromoycin solution and add 200ul each into six different beakerflask which contain 200ml LB solid medium. | + | |
| + | ===Usage=== | ||
| + | |||
| + | For testing the efficiency of paromoycin, we formulated six gradient concentrations of paromoycin solution and add 200ul each into six different beakerflask which contain 200ml LB solid medium, then we put the competent cell into six different LB plate. there is a photograph to show several monoclonal colony growing on a LB plate which has the best selected abilty of Paromoycin resistance.<b>(Fig 1)</b> | ||
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Revision as of 18:59, 16 October 2016
RBCS2 Promoter+Paromomycin+RBCS2 Terminator
We provided Paromomycin resistance under the RBCS2 promoter(BBa_K1884010)
Biology
RBCS2 promoter
Paromoycin is a protein synthesis inhibitor in nonresistant cells by binding to 16S ribosomal RNA. This broad-spectrum antibiotic soluble in water, is very similar in action to neomycin. Antimicrobial activity of paromomycin against Escherichia coli and Staphylococcus aureus has been shown.
RBCS2 terminator is Chlamydomonas reinhardtii RuBisCO small subunit 2 terminator which obtained from the pOpt_mVenus_Hyg expression plasmid provided by Chlamydomonas Resource Center.
Usage
For testing the efficiency of paromoycin, we formulated six gradient concentrations of paromoycin solution and add 200ul each into six different beakerflask which contain 200ml LB solid medium, then we put the competent cell into six different LB plate. there is a photograph to show several monoclonal colony growing on a LB plate which has the best selected abilty of Paromoycin resistance.(Fig 1)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]