Difference between revisions of "Part:BBa K2100022"
 
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<partinfo>BBa_K2100022 short</partinfo>
 
<partinfo>BBa_K2100022 short</partinfo>
  
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The construct is an entry vector containing a promoter repressible by the BM3R1 repressor.
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The construct is an entry vector containing a promoter repressible by the BM3R1 repressor. pBM3R1 consists of one repressible site upstream and four repressible sites downstream of the minimal CMV promoter. (Li et al., 2015) [1] A VP-16 Gal4 protein complex recruits the transcription machinery of the promoter. The BM3R1 repressor inhibits the activator and transcription machinery from transcribing the gene downstream of pBM3R1.  
  
 
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Latest revision as of 18:01, 16 October 2016


pENTR pBM3R1

The construct is an entry vector containing a promoter repressible by the BM3R1 repressor. pBM3R1 consists of one repressible site upstream and four repressible sites downstream of the minimal CMV promoter. (Li et al., 2015) [1] A VP-16 Gal4 protein complex recruits the transcription machinery of the promoter. The BM3R1 repressor inhibits the activator and transcription machinery from transcribing the gene downstream of pBM3R1.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Unknown
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 242
    Illegal NheI site found at 115
    Illegal NotI site found at 4
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 242
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 242
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 242
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 108
    Illegal BsaI site found at 235