Difference between revisions of "Part:BBa I712009"
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This part was submitted before the RFC[25] standard was established. Thus this part carries a modified RFC[10] pre- and suffix that changes the reading frame of the suffix in a way that, if it is ligated to a second part, it does not generate a stop codon. Although this approach makes it possible to assemble fusion proteins, the ligation to a second part, which does not carry the modified RFC[10], leads to a frameshift in the second part in a way that it is not functional anymore. | This part was submitted before the RFC[25] standard was established. Thus this part carries a modified RFC[10] pre- and suffix that changes the reading frame of the suffix in a way that, if it is ligated to a second part, it does not generate a stop codon. Although this approach makes it possible to assemble fusion proteins, the ligation to a second part, which does not carry the modified RFC[10], leads to a frameshift in the second part in a way that it is not functional anymore. | ||
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Because signal peptides are such important BioBricks we designed new signal peptide parts such as [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2170215 BBa_K2170215] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2170214 BBa_K2170214] to help other iGEM teams assembling fusion proteins. The secretion efficiency of the BioBricks was tested by Nano-Glo assay. | Because signal peptides are such important BioBricks we designed new signal peptide parts such as [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2170215 BBa_K2170215] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2170214 BBa_K2170214] to help other iGEM teams assembling fusion proteins. The secretion efficiency of the BioBricks was tested by Nano-Glo assay. | ||
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Revision as of 17:36, 16 October 2016
CD4 signal peptide; localizes to plasma membrane
Additional characterization of BBaI712009 by [http://2016.igem.org/Team:LMU-TUM_Munich Munich 2016]:
This part was submitted before the RFC[25] standard was established. Thus this part carries a modified RFC[10] pre- and suffix that changes the reading frame of the suffix in a way that, if it is ligated to a second part, it does not generate a stop codon. Although this approach makes it possible to assemble fusion proteins, the ligation to a second part, which does not carry the modified RFC[10], leads to a frameshift in the second part in a way that it is not functional anymore.
Because signal peptides are such important BioBricks we designed new signal peptide parts such as BBa_K2170215 and BBa_K2170214 to help other iGEM teams assembling fusion proteins. The secretion efficiency of the BioBricks was tested by Nano-Glo assay.
Coding region for first 25 amino acids (including start codon) of CD4 receptor which is signal peptide for plasma membrane localization.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]