Difference between revisions of "Part:BBa K2066037:Design"

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===Design Notes===
 
===Design Notes===
−
to be completed
+
placed on UNS 2/3 backbone for easy PCR and gibson assembly with universal primers.
  
 
===Source===
 
===Source===

Latest revision as of 16:59, 16 October 2016


pACT-Tet on UNS Standard


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 969
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2343
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

placed on UNS 2/3 backbone for easy PCR and gibson assembly with universal primers.

Source

pACT-Tet sequence from Amit et al. 2011 “Building Synthetic Enhancers from the Ground Up” paper,

UNS sequences from Torella et. al 2013 "Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly"

References