Difference between revisions of "Part:BBa K1997004"

 
Line 3: Line 3:
 
<partinfo>BBa_K1997004 short</partinfo>
 
<partinfo>BBa_K1997004 short</partinfo>
  
−
This is the N-terminal of split luciferase reporter. It can be used for protein-protein interaction researches.
+
This part is an integrated tool for protein-protein interaction research using split-GFP system as reporter. the "Zif268" subpart can be easily replaced using Golden Gate technique with BsaI
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 16:24, 16 October 2016


sGFP-N->Zif268->sGFP-C

This part is an integrated tool for protein-protein interaction research using split-GFP system as reporter. the "Zif268" subpart can be easily replaced using Golden Gate technique with BsaI

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 604
    Illegal AgeI site found at 688
    Illegal AgeI site found at 772
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 775
    Illegal BsaI.rc site found at 508
    Illegal BsaI.rc site found at 989