Difference between revisions of "Part:BBa K2052018"
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===Characterization=== | ===Characterization=== | ||
PCR Conformation of ButCoaT + psb1c3 | PCR Conformation of ButCoaT + psb1c3 | ||
| + | [[File:ButCoaTGelImage.jpeg|none|500px|]] | ||
Revision as of 14:53, 16 October 2016
Butyryl-CoA:acetate CoA-transferase
ButCoaT is an enzyme (Butyryl-CoA:acetate CoA-transferase) (EC 2.8.3.8) (Uniprot:C7GB37) which converts Acetyl-CoA to Butyrate and can be found in Roseburia intestinalis L1-82. The equation of the reaction (acyl-CoA:acetate CoA-transferase) (KEGG Reaction No. : R01179) is given down below :
Butanoyl-CoA + Acetate <=> Butanoic acid + Acetyl-CoA
Figure 1: Crystal Structures of Acetobacter aceti Succinyl(Butyryl)-CoA:Acetate CoA-Transferase Reveal Specificity Determinants and Illustrate the Mechanism Used by Class I CoA-Transferases.(Mullins, E.A. et al., 2012)
Butyrate
Modeling
The figure above shows the increase in ButCoAT molecules over a time span of 10 seconds.
The figure above shows the molar increase in Butyrate concentration over a time span of 10 seconds.
Design Notes
E.coli is synthesized suitable for Codon Bias.
In the expression of pet28a (expression vector) , the stop codon is removed for the addition of HisTag.
DNA Gel Analysis
Characterization
PCR Conformation of ButCoaT + psb1c3
Source
Roseburia intestinalis L1-82
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Weinstock G., Sodergren E., Clifton S., Fulton L., Fulton B., Courtney L., Fronick C., Harrison M., Strong C., Farmer C., Delahaunty K., Markovic C., Hall O., Minx P., Tomlinson C., Mitreva M., Nelson J., Hou S. Wilson R.K. (2009 , August ). UniProtKB - C7GB37 (C7GB37_9FIRM). Retrieved from http://www.uniprot.org/uniprot/C7GB37?sort=score
http://pubs.acs.org/doi/abs/10.1021/bi300957f