Difference between revisions of "Part:BBa K2132004"
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This is a modified protein, we added Histag and TEV site to the N-terminal site of HydA, and SpyCatcher to the C-terminal site of HydA.
 
This is a modified protein, we added Histag and TEV site to the N-terminal site of HydA, and SpyCatcher to the C-terminal site of HydA.
 +
For more detail information, please visit <a href="http://2016.igem.org/Team:ShanghaitechChina/Parts">http://2016.igem.org/Team:ShanghaitechChina/Parts</a> and <a href="2016.igem.org/Team:ShanghaitechChina/Hydrogen">2016.igem.org/Team:ShanghaitechChina/Hydrogen</a>.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 14:25, 16 October 2016


HydA with SpyCatcher, Histag and TEV site

This is a modified protein, we added Histag and TEV site to the N-terminal site of HydA, and SpyCatcher to the C-terminal site of HydA. For more detail information, please visit <a href="http://2016.igem.org/Team:ShanghaitechChina/Parts">http://2016.igem.org/Team:ShanghaitechChina/Parts</a> and <a href="2016.igem.org/Team:ShanghaitechChina/Hydrogen">2016.igem.org/Team:ShanghaitechChina/Hydrogen</a>.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 790
    Illegal AgeI site found at 1300
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2053
    Illegal SapI.rc site found at 663
    Illegal SapI.rc site found at 1005