Difference between revisions of "Part:BBa K2052018"
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ButCoaT is an enzyme (Butyryl-CoA:acetate CoA-transferase) (EC 2.8.3.8) (Uniprot:C7GB37) which converts Acetyl-CoA to Butyrate and can be found in Roseburia intestinalis L1-82. The equation of the reaction (acyl-CoA:acetate CoA-transferase) (KEGG Reaction No. : R01179) is given down below : | ButCoaT is an enzyme (Butyryl-CoA:acetate CoA-transferase) (EC 2.8.3.8) (Uniprot:C7GB37) which converts Acetyl-CoA to Butyrate and can be found in Roseburia intestinalis L1-82. The equation of the reaction (acyl-CoA:acetate CoA-transferase) (KEGG Reaction No. : R01179) is given down below : | ||
| + | |||
Butanoyl-CoA + Acetate <=> Butanoic acid + Acetyl-CoA | Butanoyl-CoA + Acetate <=> Butanoic acid + Acetyl-CoA | ||
[[File:ButCoaTStructure.gif|none|frame|500px]] | [[File:ButCoaTStructure.gif|none|frame|500px]] | ||
| − | Figure 1. | + | Figure 1: Crystal Structures of Acetobacter aceti Succinyl(Butyryl)-CoA:Acetate CoA-Transferase Reveal Specificity Determinants and Illustrate the Mechanism Used by Class I CoA-Transferases.(Mullins, E.A. et al., 2012) |
| − | + | ||
| − | + | ==Modeling== | |
[[File:ButCoaTModeling.jpeg|none|700px|caption]] | [[File:ButCoaTModeling.jpeg|none|700px|caption]] | ||
| Line 17: | Line 17: | ||
The figure above shows the molar increase in Butyrate concentration over a time span of 10 seconds. | The figure above shows the molar increase in Butyrate concentration over a time span of 10 seconds. | ||
| − | + | ==Design Notes== | |
E.coli is synthesized suitable for Codon Bias. | E.coli is synthesized suitable for Codon Bias. | ||
| Line 23: | Line 23: | ||
In the expression of pet28a (expression vector) , the stop codon is removed for the addition of HisTag. | In the expression of pet28a (expression vector) , the stop codon is removed for the addition of HisTag. | ||
| + | ==DNA Gel Analysis== | ||
===Characterization=== | ===Characterization=== | ||
| − | + | ==Source== | |
Roseburia intestinalis L1-82 | Roseburia intestinalis L1-82 | ||
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<!-- --> | <!-- --> | ||
| − | + | ==References== | |
| + | http://pubs.acs.org/doi/abs/10.1021/bi300957f | ||
Revision as of 14:22, 16 October 2016
Butyryl-CoA:acetate CoA-transferase
ButCoaT is an enzyme (Butyryl-CoA:acetate CoA-transferase) (EC 2.8.3.8) (Uniprot:C7GB37) which converts Acetyl-CoA to Butyrate and can be found in Roseburia intestinalis L1-82. The equation of the reaction (acyl-CoA:acetate CoA-transferase) (KEGG Reaction No. : R01179) is given down below :
Butanoyl-CoA + Acetate <=> Butanoic acid + Acetyl-CoA
Figure 1: Crystal Structures of Acetobacter aceti Succinyl(Butyryl)-CoA:Acetate CoA-Transferase Reveal Specificity Determinants and Illustrate the Mechanism Used by Class I CoA-Transferases.(Mullins, E.A. et al., 2012)
Modeling
The figure above shows the increase in ButCoAT molecules over a time span of 10 seconds.
The figure above shows the molar increase in Butyrate concentration over a time span of 10 seconds.
Design Notes
E.coli is synthesized suitable for Codon Bias.
In the expression of pet28a (expression vector) , the stop codon is removed for the addition of HisTag.
DNA Gel Analysis
Characterization
Source
Roseburia intestinalis L1-82
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
http://pubs.acs.org/doi/abs/10.1021/bi300957f