Difference between revisions of "Part:BBa K2012018"
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<partinfo>BBa_K2012018 short</partinfo> | <partinfo>BBa_K2012018 short</partinfo> | ||
| − | this generator is altered from BBa_K819010 created by Peking University.Its previous promotor , | + | this generator is altered from BBa_K819010 created by Peking University.Its previous promotor , J23113,is replaced by a medium promotor J23106. This generator is constructed to detect the relationship between different promoters' strength with their swarming ability. A series of Biobricks were constructed as BBa_K2012017[https://parts.igem.org/Part:BBa_K2012017], BBa_K2012020[https://parts.igem.org/Part:BBa_K2012020] |
| − | <html><img src="https://static.igem.org/mediawiki/parts/ | + | <p>The swarming plate of different <i>E.coli</i> K12 cheZ lacking strain, CL1, transformed with our constructed plamids</p> |
| − | </html> | + | <html><img src="https://static.igem.org/mediawiki/parts/0/00/T--HZAU-China--swarming_plate_of_CheZ.jpg" style="width:900px;margin-left:0%;"/></html> |
| + | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 13:47, 16 October 2016
A medium CheZ generator
this generator is altered from BBa_K819010 created by Peking University.Its previous promotor , J23113,is replaced by a medium promotor J23106. This generator is constructed to detect the relationship between different promoters' strength with their swarming ability. A series of Biobricks were constructed as BBa_K2012017[1], BBa_K2012020[2]
The swarming plate of different E.coli K12 cheZ lacking strain, CL1, transformed with our constructed plamids
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]