Difference between revisions of "Part:BBa K2012017"

Revision as of 13:42, 16 October 2016

A strong generator of CheZ

this generator is altered from BBa_K819010[1] created by Peking University.Its previous promoter , J23117,is replaced by a much stronger promotor J23101. This generator is constructed to detect the relationship between different promoters' strength with their swarming ability. A series of Biobricks were constructed as BBa_K2012018[2], BBa_K2012020[3]

The swarming plate of different E.coli K12 cheZ lacking strain, CL1, transformed with our constructed plamids

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 this generator is altered from BBa_K819010[https://parts.igem.org/Part:BBa_K819010] created by Peking University.Its previous promoter , J23117,is replaced by a much stronger promotor J23101. This generator is constructed to detect the relationship between different promoters' strength  with their swarming ability. A series of Biobricks were constructed as BBa_K2012018[https://parts.igem.org/Part:BBa_K2012018], BBa_K2012020[https://parts.igem.org/Part:BBa_K2012020]
-<html><img src="https://static.igem.org/mediawiki/parts/7/7b/T--HZAU-China--CL1_control.jpg" style="width:600px;margin-left:5%;"/></html>
+<html><img src="https://static.igem.org/mediawiki/parts/0/00/T--HZAU-China--swarming_plate_of_CheZ.jpg" style="width:1000px;margin-left:0%;"/></html>
 <p>The swarming plate of different <i>E.coli</i> K12 cheZ lacking strain, CL1, transformed with our constructed plamids</p>