Difference between revisions of "Part:BBa K2052018"
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<partinfo>BBa_K2052018 short</partinfo> | <partinfo>BBa_K2052018 short</partinfo> | ||
+ | ButCoaT is an enzyme (Butyryl-CoA:acetate CoA-transferase) (EC 2.8.3.8) (Uniprot:C7GB37) which converts Acetyl-CoA to Butyrate and can be found in Roseburia intestinalis L1-82. The equation of the reaction (acyl-CoA:acetate CoA-transferase) (KEGG Reaction No. : R01179) is given down below : | ||
+ | Butanoyl-CoA + Acetate <=> Butanoic acid + Acetyl-CoA | ||
+ | [[File:ButCoaTStructure.gif|none|frame|500px]] | ||
+ | Figure 1. | ||
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− | === | + | ===Modeling=== |
− | + | [[File:ButCoaTModeling.jpeg|none|700px|caption]] | |
− | + | The figure above shows the increase in ButCoAT molecules over a time span of 10 seconds. | |
+ | [[File:ButyrateModeling.jpeg|none|700px|caption]] | ||
+ | The figure above shows the molar increase in Butyrate concentration over a time span of 10 seconds. | ||
+ | ===Design Notes=== | ||
− | + | E.coli is synthesized suitable for Codon Bias. | |
+ | In the expression of pet28a (expression vector) , the stop codon is removed for the addition of HisTag. | ||
− | === | + | ===Characterization=== |
+ | |||
+ | ===Source=== | ||
+ | |||
+ | Roseburia intestinalis L1-82 | ||
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<partinfo>BBa_K2052018 parameters</partinfo> | <partinfo>BBa_K2052018 parameters</partinfo> | ||
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===References=== | ===References=== | ||
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Revision as of 13:37, 16 October 2016
Butyryl-CoA:acetate CoA-transferase
ButCoaT is an enzyme (Butyryl-CoA:acetate CoA-transferase) (EC 2.8.3.8) (Uniprot:C7GB37) which converts Acetyl-CoA to Butyrate and can be found in Roseburia intestinalis L1-82. The equation of the reaction (acyl-CoA:acetate CoA-transferase) (KEGG Reaction No. : R01179) is given down below : Butanoyl-CoA + Acetate <=> Butanoic acid + Acetyl-CoA
Figure 1.
Modeling
The figure above shows the increase in ButCoAT molecules over a time span of 10 seconds.
The figure above shows the molar increase in Butyrate concentration over a time span of 10 seconds.
Design Notes
E.coli is synthesized suitable for Codon Bias.
In the expression of pet28a (expression vector) , the stop codon is removed for the addition of HisTag.
Characterization
Source
Roseburia intestinalis L1-82
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]