Difference between revisions of "Part:BBa K1943000:Design"

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This biobrick is constructed through 2 steps:
 
This biobrick is constructed through 2 steps:
  
−
# Do double enzyme digestion (EcoRI & SpeI for BBa_K592012, EcoRI & XbaI for BBa_B0015) and do gel extraction, ligation, transformation, picking up single colonies, LB broth incubation, plasmid extraction and gel electrophoresis verification.
+
# Do double enzyme digestion (EcoRI & SpeI for BBa_K1033910, EcoRI & XbaI for BBa_B0015) and do gel extraction, ligation, transformation, picking up single colonies, LB broth incubation, plasmid extraction and gel electrophoresis verification.
 
# Do double enzyme digestion (XbaI & PstI for Step 1 product, SpeI & PstI for BBa_K880005) and do gel extraction, ligation, transformation, picking up single colonies, LB broth incubation, plasmid extraction and gel electrophoresis verification.
 
# Do double enzyme digestion (XbaI & PstI for Step 1 product, SpeI & PstI for BBa_K880005) and do gel extraction, ligation, transformation, picking up single colonies, LB broth incubation, plasmid extraction and gel electrophoresis verification.
  

Revision as of 13:21, 16 October 2016


fwYellow, yellow chromoprotein reporter system (Strong Promoter, Strong RBS)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 170
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 547


Design Notes

This biobrick is constructed through 2 steps:

  1. Do double enzyme digestion (EcoRI & SpeI for BBa_K1033910, EcoRI & XbaI for BBa_B0015) and do gel extraction, ligation, transformation, picking up single colonies, LB broth incubation, plasmid extraction and gel electrophoresis verification.
  2. Do double enzyme digestion (XbaI & PstI for Step 1 product, SpeI & PstI for BBa_K880005) and do gel extraction, ligation, transformation, picking up single colonies, LB broth incubation, plasmid extraction and gel electrophoresis verification.

Source

The following list is the Biobricks we’ve used in construction and their detailed design information.

Part Type Discription Backbone Location on the plate
BBa_K880005 Promoter + RBS Strong Promoter + Strong RBS pSB1C3 3F 2016 Kit Plate 2
BBa_K608007 Promoter + RBS Medium Promoter + Weak RBS pSB1C3 5G 2016 Kit Plate 1
BBa_K592012 Chromoprotein eforRed, red chromoprotein pSB1C3 15I 2016 Kit Plate 6
BBa_K1033919 Chromoprotein gfasPurple, purplr chromoprotein pSB1C3 9K 2016 Kit Plate 6
BBa_K1033910 Chromoprotein fwYellow, yellow chromoprotein pSB1C3 6K 2016 Kit Plate 4
BBa_K1033932 Chromoprotein spisPink, pink chromoprotein pSB1C3 11K 2016 Kit Plate 6
BBa_B0015 Terminator Double terminator pSB1C3 3F 2016 Kit Plate 3

References