Difference between revisions of "Part:BBa K1985003"

Revision as of 12:22, 16 October 2016

mamP, his-tagged, signal sequence cleaved

This part is an differentiation on Part:BBa_K1985000.

Usage and Biology

This part has a his-tag included so was used to purify the expressed mamP protein. MamP is usually targeted to the membrane, however in this part the targeting sequence has been cleaved and it should instead be targeted to the periplasm.

It was expressed, purified and then exposed to iron.

For more information on its biology and usage, see part BBa_K1985000.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 517
1000
COMPATIBLE WITH RFC[1000]

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 This part is an differentiation on Part:[https://parts.igem.org/Part:BBa_K1985000 BBa_K1985000].
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
-This part has a his-tag included so was used to purify the expressed mamP protein. MamP is usually targeted to the membrane, however in this part the targeting sequence has been cleaved and it should instead be targeted to the periplasm. For more information on its biology and usage, see part BBa_K1985000.
+This part has a his-tag included so was used to purify the expressed mamP protein. MamP is usually targeted to the membrane, however in this part the targeting sequence has been cleaved and it should instead be targeted to the periplasm.
+It was expressed, purified and then exposed to iron.
+For more information on its biology and usage, see part BBa_K1985000.
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