Difference between revisions of "Part:BBa K1231000:Experience"

Revision as of 11:41, 16 October 2016

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Applications of BBa_K1231000

User Reviews

UNIQdde6eee0acbd198f-partinfo-00000000-QINU

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iGEM Dundee 2016

The function of this part was characterised in the hope of designing a pH-responsive system. We hooked up this asr promomoter to an excellent reporter system (BBa_E0840) and characterised its activity under this new composite biobrick (BBa_K1962014). The system showed some pH-dependent activity.

We also hooked up the asr promoter to biobricks encoding immunity proteins towards colicins. This should give some degree of regulation of transcription of the Im-Ia immunity protein (standard biobrick BBa_K1962001 and composite part BBa_K1962015) and the Im-E3 immunity protein (standard biobrick BBa_K1962004 and composite part BBa_K1962016).

UNIQdde6eee0acbd198f-partinfo-00000008-QINU

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 The function of this part was characterised in the hope of designing a pH-responsive system. We hooked up this <i>asr</i> promomoter to an excellent reporter system (<partinfo>BBa_E0840</partinfo>) and characterised its activity under this new composite biobrick (<partinfo>BBa_K1962014</partinfo>). The system showed some pH-dependent activity.
-We also hooked up the <i>asr</i> promoter to biobricks encoding immunity proteins towards colicins. This should give some degree of regulation of transcription of the Im-Ia immunity protein (standard biobrick <partinfo> BBa_K1962001</partinfo> and composite part <partinfo>BBa_K1962015</partinfo>) and the Im-E3 immunity protein (standard biobrick <partinfo>BBa_K1962004</partinfo> and composite part <partinfo>BBa_K1962016</partinfo>).
+We also hooked up the <i>asr</i> promoter to biobricks encoding immunity proteins towards colicins. This should give some degree of regulation of transcription of the Im-Ia immunity protein (standard biobrick <partinfo>BBa_K1962001</partinfo> and composite part <partinfo>BBa_K1962015</partinfo>) and the Im-E3 immunity protein (standard biobrick <partinfo>BBa_K1962004</partinfo> and composite part <partinfo>BBa_K1962016</partinfo>).
 |}