Difference between revisions of "Part:BBa K2020042:Design"

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===References===
 
===References===
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*Zhang et al, 2002, Structure-based design of mutant Methanococcus jannaschii tyrosyl-tRNA synthetase for incorporation of O-methyl-L-tyrosine
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#Zhang et al, 2002, Structure-based design of mutant Methanococcus jannaschii tyrosyl-tRNA synthetase for incorporation of O-methyl-L-tyrosine
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*Kobayashi et al, 2003, Structural basis for the orthogonal tRNA specificities of tyrosyl-tRNA synthetasees for genetic code expansion
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#Kobayashi et al, 2003, Structural basis for the orthogonal tRNA specificities of tyrosyl-tRNA synthetasees for genetic code expansion

Revision as of 10:41, 16 October 2016


Mj-tRNA with amber anticodon for incorporating ncAA in E.coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

tRNA has 77 amino acids. The ncAA will be attached to the 3' end. On the contrary to aminoacylation with natural amino acids, the acceptor minihelix alone is a substrate for aminoacylation with ncAA's.

Source

wild type tyrosyl Methanococcus janaschii tRNA:Synthetase-pair with anticodon changed to Amber Anticodon CTA. Obtained via iGEM Team 2014 Austin, Texas.

References

  1. Zhang et al, 2002, Structure-based design of mutant Methanococcus jannaschii tyrosyl-tRNA synthetase for incorporation of O-methyl-L-tyrosine
  2. Kobayashi et al, 2003, Structural basis for the orthogonal tRNA specificities of tyrosyl-tRNA synthetasees for genetic code expansion