Difference between revisions of "Part:BBa K1884001"
Nllenwalker (Talk | contribs) (→Usage) |
Nllenwalker (Talk | contribs) (→Usage) |
||
| Line 11: | Line 11: | ||
===Usage=== | ===Usage=== | ||
| − | To make this parts RFC 10 competible, we use Site-directed mutagenesis to mutate the old sequence | + | To make this parts RFC 10 competible, we use Site-directed mutagenesis to mutate the old sequence so that none of these four Restriction enzyme(EcoR1, Xba1， Sep1， Pst1) can digest this gene. |
| + | |||
| + | <html> | ||
| + | |||
| + | <figure style="text-align: center"><img style="width:70%" src="https://static.igem.org/mediawiki/2015/a/a0/ShTERT_sequence.png"/><figcaption style="text-align:center"> | ||
| + | |||
Revision as of 10:16, 16 October 2016
Gal4BD-CIB1
Cryptochrome-interacting basic-helix-loop-helix 1(CIB1) is a protein-coded gene. The product of this gene expression is a basic helix-loop-helix (bHLH) protein which would interact with cryptochrome 2 (CRY2), a blue light stimulated photoreceptor, when exposed to blue light. This part is a Gal4 DNA binding domain fused to C terminus of CIB1.
Biology
This fusion protein is for use in a yeast-two-hybrid system,and a Gal4 DNA binding domian fused to its C terminus. In order to control DNA transcription by blue light, the system is based on a two-hybrid interaction in which a light-mediated protein brings together two halves of a split transcription factor. CRY2 will disconnected with CIB1 in the dark and halt the DNA transcription.
Usage
To make this parts RFC 10 competible, we use Site-directed mutagenesis to mutate the old sequence so that none of these four Restriction enzyme(EcoR1, Xba1， Sep1， Pst1) can digest this gene.
