Difference between revisions of "Part:BBa K2040100:Design"
Chilam Poon (Talk | contribs) (→Design Notes) |
Chilam Poon (Talk | contribs) (→Design Notes) |
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===Design Notes=== | ===Design Notes=== | ||
| + | Two illegal cutsites (XbaI and SpeI) were subsequently removed via site-directed mutagenesis. | ||
===Source=== | ===Source=== | ||
Revision as of 10:05, 16 October 2016
PMcl1, hemolymph inducible promoter from Metarhizium anisopliae
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1673
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 853
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1229
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1179
Illegal SapI site found at 1130
Design Notes
Two illegal cutsites (XbaI and SpeI) were subsequently removed via site-directed mutagenesis.
Source
It was isolated from the genomic DNA of our chassis organism Metarhizium anisopliae ARSEF549.