Difference between revisions of "Part:BBa K2040100:Experience"

Revision as of 07:42, 16 October 2016

Applications of BBa_K2040100

We used forward primer 5' ACGTCCTGCAGAATCATGCAGCGCTATGAG 3' & reverse primer 5' ATAAGCGGCCGCCATGATGGTCTAGGGAACG 3' to extract PMcl1 and its 5' untranslated region (99bp downstream the promoter) from genomic DNA of Metarhizium anisopliae. The whole length is 2772bp.

Figure1. Amplify PMcl1 from gDNA

Then we digested the DNA fragment with NotI and PstI in order to insert it into the backbone. However, when we ran gel electrophoresis to check the digestion result, we found that there is still one PstI restriction site inside the PMcl1 whcih is the one we did not know when we designed.

User Reviews

UNIQ668170afbf1a6bda-partinfo-00000000-QINU UNIQ668170afbf1a6bda-partinfo-00000001-QINU

@@ Line 1: / Line 1: @@
 __NOTOC__
-This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
-how you used this part and how it worked out.
 ===Applications of BBa_K2040100===
-We used primer
+We used
+forward primer
 ' ACGTCCTGCAGAATCATGCAGCGCTATGAG 3'
+& reverse primer
 ' ATAAGCGGCCGCCATGATGGTCTAGGGAACG 3'
-to amplify PMcl1 and XXbp 5' untranslated region downstream the promoter from genomic DNA of ''Metarhizium anisopliae''.
+to extract PMcl1 and its 5' untranslated region (99bp downstream the promoter) from genomic DNA of ''Metarhizium anisopliae''. The whole length is 2772bp.
+[[Image:PMcl1_PCR.png|left|250px|thumb|Figure1. Amplify PMcl1 from gDNA]]
+Then we digested the DNA fragment with NotI and PstI in order to insert it into the backbone. However, when we ran gel electrophoresis to check the digestion result, we found that there is still one PstI restriction site inside the PMcl1 whcih is the one we did not know when we designed.
-[]
 ===User Reviews===