Difference between revisions of "Part:BBa K1898150:Design"

 
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<partinfo>BBa_K1898150 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1898150 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
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this DNA is synthesized by IDT.  
 
this DNA is synthesized by IDT.  
 +
 +
The following primers are designed and used to move the DNA from IDT to iGEM Biobrick:
 +
  
 
===References===
 
===References===

Revision as of 06:46, 16 October 2016


Strong promoter + Strong RBS + CH25H + 10x His tag + Double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

We made sure that there was no Ecor1, Xbal, Spel1, and Pst1 cutting sites in the DNA.


Source

this DNA is synthesized by IDT.

The following primers are designed and used to move the DNA from IDT to iGEM Biobrick:


References