Difference between revisions of "Part:BBa J45017"

Revision as of 01:16, 20 July 2007

isochorismate pyruvate-lyase and isochorismate synthase (pchBA); converts chorismate to salicylate

PchBA codes for proteins used to generate salicylic acid (the precursor to methyl salicylate or wintergreen scent) from the common metabolite, chorismate. The two genes are at the downstream end of the PchDCBA operon that Pseudomonas species use to encode enzymes that make pyochelin, a molecule that helps them acquire iron. This part doesn't make a fusion protein, even though the coding regions overlap; the RBS for PchA is located at the end of the coding region of PchB.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 307
Illegal NgoMIV site found at 711
Illegal NgoMIV site found at 836
Illegal NgoMIV site found at 847
Illegal NgoMIV site found at 1128
Illegal NgoMIV site found at 1596
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_J45017 short</partinfo>
-PchBA codes for proteins used to generate salicylic acid (the precursor to methyl salicylate or wintergreen scent) from the common metabolite, chorismate. The two genes are at the downstream end of the PchDCBA operon that ''Pseudomonas'' species used to encode enzymes that produce pyochelin, a molecule that they need to acquire iron. This part doesn't make a fusion protein, even though the coding regions overlap; the RBS for PchA is located at the end of the coding region of PchB.
+PchBA codes for proteins used to generate salicylic acid (the precursor to methyl salicylate or wintergreen scent) from the common metabolite, chorismate. The two genes are at the downstream end of the PchDCBA operon that ''Pseudomonas'' species use to encode enzymes that make pyochelin, a molecule that helps them acquire iron. This part doesn't make a fusion protein, even though the coding regions overlap; the RBS for PchA is located at the end of the coding region of PchB.
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