Difference between revisions of "Part:BBa K1898150"

 
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<partinfo>BBa_K1898150 short</partinfo>
 
<partinfo>BBa_K1898150 short</partinfo>
  
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BBa_K880005 consists of strong promoter and strong rbs to maximize protein production. This construct codes for ch25h, a catalyst for the conversion of cholesterol to 25 hydroxycholesterol, and 10x histidine tag for protein purification. BBa__B0015 is a double terminator used to stop transcription.  
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BBa_K880005 consists of strong promoter and strong rbs to maximize protein production. This construct codes for CH25H (cholesterol 25-Hydroxylase) and a 10x Histidine Tag. CH25H is a catalyst that will make 25 hydroxycholesterol and the 10x Histidine tag is used for protein purification. BBa__B0015 is a double terminator used to stop transcription.  
  
 
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<partinfo>BBa_K1898150 parameters</partinfo>
 
<partinfo>BBa_K1898150 parameters</partinfo>
 
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==Gel Pictures==
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PCR was set up after moving the synthesized DNA from IDT to iGEM BIobrick. The expected PCR band sizes are ~1.3 kb.
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==Sequencing==
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The following sequence was sent to IDT for DNA syntehsis:
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This quality report came back from IDT:

Revision as of 06:45, 16 October 2016


Strong promoter + Strong RBS + CH25H + 10x His tag + Double terminator

BBa_K880005 consists of strong promoter and strong rbs to maximize protein production. This construct codes for CH25H (cholesterol 25-Hydroxylase) and a 10x Histidine Tag. CH25H is a catalyst that will make 25 hydroxycholesterol and the 10x Histidine tag is used for protein purification. BBa__B0015 is a double terminator used to stop transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Gel Pictures

PCR was set up after moving the synthesized DNA from IDT to iGEM BIobrick. The expected PCR band sizes are ~1.3 kb.

Sequencing

The following sequence was sent to IDT for DNA syntehsis:


This quality report came back from IDT: