Difference between revisions of "Part:BBa K1898100"

Revision as of 05:47, 16 October 2016

ch25h, cholesterol 25 hydroxylase

This DNA codes for ch25h, cholesterol 25-hydroxylase, that catalyzes the formation of 25 hydroxycholesterol from cholesterol.

Usage and Biology

25 hydroxycholesterol (25 HC) has been reported to have a range of effects on the immune system, including the suppression of production of IgA by B cells and inducing the migration of human peripheral blood monocytes [1]. 25 HC is also found to be able to reverse protein aggregation and restore solubility to the proteins [2]. In our project, we aim to deliver CH25H to the lens, where 25 HC will be produced and reverse aggregated lens protein. Aged-induced cataracts occur when lens proteins get oxidized and clump together. the increase of 25HC in the lens will help to treat cataracts.

[1] Diczfalusy, U. (2013). On the formation and possible biological role of 25-hydroxycholesterol. Biochimie, 95(3), 455-460. doi:10.1016/j.biochi.2012.06.016 [2] Makley, L. N., McMenimen, K. A., DeVree, B. T., Goldman, J. W., McGlasson, B. N., Rajagopal, P., Dunyak, B.M., McQuade, T.J., Thompson, A.D., Sunahara, R., Klevit, R.E., Andley, U.P., and Gestwicki, J.E. (2015). Pharmacological chaperone for α-crystallin partially restores transparency in cataract models. Science, 350(6261), 674-677.

Sequence and Features