Difference between revisions of "Part:BBa K2012013"
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<p>We have proved the function of GFP and CheZ by observing it under the fluorescence microscope and transforming it into a cheZ lacking strain to process swarming assay seperately<br/> the image are as following </p>
 
<p>We have proved the function of GFP and CheZ by observing it under the fluorescence microscope and transforming it into a cheZ lacking strain to process swarming assay seperately<br/> the image are as following </p>
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<img src="https://static.igem.org/mediawiki/parts/7/7b/T--HZAU-China--CL1_control.jpg" style="width:400px;margin-left:5%;"/>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 05:32, 16 October 2016


Strong CheZ generator with a fusion GFP reporter

This generator contain promotor J23101 ,RBS from BBa_J61100,functionaal chemotaxis gene CheZ,reporter gene GFP from BBa_E0040 and a 18bp-GS linker between two functional domain.It can generate CheZ with a high ouIt is used to detect the relationship of CheZ under different promotor strength and its swimming ability.

We have proved the function of GFP and CheZ by observing it under the fluorescence microscope and transforming it into a cheZ lacking strain to process swarming assay seperately
the image are as following

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 715
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1362