Difference between revisions of "Part:BBa K1871010"

Revision as of 03:12, 16 October 2016

Coding Sequence AMY1

This is an alpha amylase gene obtained from Bacillus amyloliquefaciens.

Native Alpha Amylase Secretion Sequence

This is the Bacillus amyloliquefaciens alpha amylase signal sequence, which directs the protein to be secreted.

Alpha Amylase

Obtained from Bacillus amyloliquefaciens, α-Amylase is a protein enzyme that hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen.

ADH1 Terminator

This part is the terminator region from yeast alcohol dehydrogenase (ADH1) gene. This stops the RNA polymerase from transcribing the RNA sequence.

Usage and Biology

This part was proved functional by the 2015 BRHS iGEM Team. Please click the below link to see their results. http://2015.igem.org/Team:BroadRun-NorthernVA/Results

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 8
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1436
Illegal NgoMIV site found at 1451
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
-This is an alpha amylase gene obtained from ''Bacillus amyloliquefaciens''. It is flanked by a Kozak sequence and ADH1 terminator but lacks a promoter.
+This is an alpha amylase gene obtained from ''Bacillus amyloliquefaciens''.
-====Kozak Sequence====
-This part initiates translation from eukaryotic mRNA and is cloned between a promoter and coding region to facilitate translation.
 ====Native Alpha Amylase Secretion Sequence====
@@ Line 20: / Line 19: @@
-<!-- Add more about the biology of this part here
 ====Usage and Biology====
+This part was proved functional by the 2015 BRHS iGEM Team. Please click the below link to see their results.
+http://2015.igem.org/Team:BroadRun-NorthernVA/Results
-<!-- -->
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K1871010 SequenceAndFeatures</partinfo>