Difference between revisions of "Part:BBa K1949030:Design"

(Source)
Line 15: Line 15:
 
===Source===
 
===Source===
  
The gene sequence was isolated from E. coli BL21
+
The gene sequence was isolated from E. coli BL21<br>
 +
 
 +
===Materials and Methods===
 +
 
 +
=====Construction=====
 +
 
 +
=====Assay protocol=====
 +
 
 +
 
 +
 
 +
 
  
  
 
===References===
 
===References===

Revision as of 02:16, 16 October 2016


yafO


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 335
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

yafO gene fragment amplified by PCR contains XbaI site at N-terminal and SpeI and PstI site at C-terminal. Both terminals were cut by respective restriction enzymes and the fragment used for ligation with iGEM plasmid vectors.

fwd:5’-AAATCTAGATGCGGGTATTCAAAACAAAACTTA-3’
rev:5’-AAACTGCAGCGGCCGCTACTAGTATTATTAAAAACGCATGCGAAACGCTTCTGCCA-3’


Source

The gene sequence was isolated from E. coli BL21

Materials and Methods

Construction
Assay protocol

===References===