Difference between revisions of "Part:BBa K2042015:Design"
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===Design Notes=== | ===Design Notes=== | ||
| + | A BamHI site was placed between the promoter and the RBS in order allow expression with other promoter | ||
This part was introduced in pSEVA424 plasmid in order to increase the lactate fraction in Pseudomonas putida. | This part was introduced in pSEVA424 plasmid in order to increase the lactate fraction in Pseudomonas putida. | ||
Latest revision as of 01:20, 16 October 2016
LDH* Generator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 45
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 699
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
A BamHI site was placed between the promoter and the RBS in order allow expression with other promoter This part was introduced in pSEVA424 plasmid in order to increase the lactate fraction in Pseudomonas putida.
Source
The LDH* were synthesized from the genomic sequence of Lactobacillus delbrueckii 11842