Difference between revisions of "Part:BBa K1936001:Design"

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Tom5 yeast sequence:
 
Tom5 yeast sequence:
 
http://www.ebi.ac.uk/ena/data/view/AY558369
 
http://www.ebi.ac.uk/ena/data/view/AY558369
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−
 
 
eGFP sequence:
 
eGFP sequence:
−
 
  
 
LOV2 sequence:
 
LOV2 sequence:

Revision as of 00:24, 16 October 2016


Tom5-eGFP-LOV2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 33
    Illegal BamHI site found at 712
    Illegal XhoI site found at 515
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1226


Design Notes

ALERT !!!Under construction !!


Source

Note: all used sequences except for linkers where codon optimized for use in H.sapiens. Therefore base sequences may not match with mentioned sources.


Tom5 yeast sequence: http://www.ebi.ac.uk/ena/data/view/AY558369 eGFP sequence:

LOV2 sequence: (double mutant)

References