Difference between revisions of "Part:BBa K1951009"
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==Design summary==
 
==Design summary==
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We find the sequence of the flagellin from <i>Desulfovibrio vulgaris</i> in
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We found the sequence in online databases, and after processed it ordered it to IDT.
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We assess the production of the protein by a SDS page/comassie test. We made a FliC mutant by transduction using phage P1 in a E. Coli W3110 strain.
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The cloning was made fisrly in the pSB1C3 vector thanks to SLIC oligo designed by our team.
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==Experience summary==
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once sequencing data indicated that the cloning and transformation was successful, we assessed the production of the protein by a SDS page/comassie test. COOMASSIE
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However, no functionality test as swimming test has been carried out with this biobrick contrary to the [https://parts.igem.org/Part:BBa_K1951008#Experience_summary FliC protein] from ''E .coli''
  
 
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Revision as of 22:02, 15 October 2016


FliC Desulfovibrio producer

FliC, the main swimming protein (K1951006 + K880005)

General

Flagellin C (FliC) protein from Desulfovibrio vulgaris strain is the main protein constitutive of the flagellum filament and is involved to promote bacterial swimming[1]. This sequence is conserved in many bacterial strains as the capacity of swimming given by the flagellum confers a great selective advantage.

Metal Biosorption Capacity

It has been demonstrated that Flagellin has the ability to adsorb precious metal on its surface such as platinum, palladium gold [2]...

Immune response capacity

The propensity of the immune response to flagellin may be explained by two facts:

  • Flagellin is an extremely abundant protein in flagellated bacteria.
  • There exists a specific innate immune receptor that recognizes flagellin, Toll-like receptor 5 (TLR5). [3]

.

Design summary

We found the sequence in online databases, and after processed it ordered it to IDT. The cloning was made fisrly in the pSB1C3 vector thanks to SLIC oligo designed by our team.

Experience summary

once sequencing data indicated that the cloning and transformation was successful, we assessed the production of the protein by a SDS page/comassie test. COOMASSIE However, no functionality test as swimming test has been carried out with this biobrick contrary to the FliC protein from E .coli

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 362
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 461


  1. ↑ Capeness & al. 2015, http://eprints.nottingham.ac.uk/27979/1/Michael%20Capeness%20-%20Thesis%20-%20PDF.pdf
  2. ↑ Deplanche & al., 2007 http://onlinelibrary.wiley.com/doi/10.1002/bit.21688/abstract.
  3. ↑ Kathrani A. & al, 2012 http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0030117)