Difference between revisions of "Part:BBa K2042012:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | We inserted non-coding scar sequences after RBS witch is close to the gene to prevent missing initial codons during translation. | |
===Source=== | ===Source=== | ||
Revision as of 21:50, 15 October 2016
RBS + PhaC_sp. MBEL 6-19
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1073
Illegal NgoMIV site found at 1352
Illegal AgeI site found at 148
Illegal AgeI site found at 461 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We inserted non-coding scar sequences after RBS witch is close to the gene to prevent missing initial codons during translation.
Source
Synthetic synthesis