Difference between revisions of "Part:BBa K1951009"
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==Design summary==
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We find the sequence of the flagellin from <i>Desulfovibrio vulgaris</i> in 
 
We assess the production of the protein by a SDS page/comassie test. We made a FliC mutant by transduction using phage P1 in a E. Coli W3110 strain.  
 
We assess the production of the protein by a SDS page/comassie test. We made a FliC mutant by transduction using phage P1 in a E. Coli W3110 strain.  
  

Revision as of 21:46, 15 October 2016


FliC Desulfovibrio producer

FliC, the main swimming protein (K1951006 + K880005)

General

Flagellin C (FliC) protein from Desulfovibrio vulgaris strain is the main protein constitutive of the flagellum filament and is involved to promote bacterial swimming[1]. This sequence is conserved in many bacterial strains as the capacity of swimming given by the flagellum confers a great selective advantage.

Metal Biosorption Capacity

It has been demonstrated that Flagellin has the ability to adsorb precious metal on its surface such as platinum, palladium gold [2]...

Immune response capacity

The propensity of the immune response to flagellin may be explained by two facts:

  • Flagellin is an extremely abundant protein in flagellated bacteria.
  • There exists a specific innate immune receptor that recognizes flagellin, Toll-like receptor 5 (TLR5). [3]

.

Design summary

We find the sequence of the flagellin from Desulfovibrio vulgaris in We assess the production of the protein by a SDS page/comassie test. We made a FliC mutant by transduction using phage P1 in a E. Coli W3110 strain.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 362
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 461


  1. ↑ Capeness & al. 2015, http://eprints.nottingham.ac.uk/27979/1/Michael%20Capeness%20-%20Thesis%20-%20PDF.pdf
  2. ↑ Deplanche & al., 2007 http://onlinelibrary.wiley.com/doi/10.1002/bit.21688/abstract.
  3. ↑ Kathrani A. & al, 2012 http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0030117)