Difference between revisions of "Part:BBa K2065002"

Revision as of 21:35, 15 October 2016

T14-3-3 (S71L) - T14-3-3

Usage and Biology

Sequence

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1234
Illegal EcoRI site found at 1389
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1234
Illegal EcoRI site found at 1389
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1234
Illegal EcoRI site found at 1389
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1234
Illegal EcoRI site found at 1389
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1234
Illegal EcoRI site found at 1389
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 937

Bioluminescence confirmation

LargeBiT-GGS10-CT52 Statistics

Protein Specifications
General Information	Number of amino acids		514
	Molecular weight		56797.79
	Theoretical pi		4.96
	Extinction coefficient		55030 of 54780
	Formula		C₂₄₈₀H₃₉₅₀N₆₈₀O₈₀₈S₁₈
	Total numbers of atons		7936
Amino Acid Composition	Amino Acid	Frequency	Percentage(%)
	Ala(A)	53	10.3
	Arg(R	28	5.4
	Asn(N)	22	4.3
	Asp(D)	22	4.3
	Cys(C)	4	0.8
	Gln(Q)	12	2.3
	Glu(E)	60	11.7
	Gly(G)	39	7.6
	His(H)	6	1.2
	Ile(I)	28	5.4
	Leu(L)	53	10.3
	Lys(K)	32	6.2
	Met(M)	14	2.7
	Phe(F)	12	2.3
	Pro(P)	10	1.9
	Ser(S)	49	9.5
	Thr(T)	24	4.7
	Trp(W)	4	0.8
	Tyr(T)	22	4.3
	Val(V)	20	3.9
	Pyl(O)	0	0.0
	Sec(U)	0	0.0

@@ Line 44: / Line 44: @@
 					<td style="font-weight:bold;" rowspan="6">General Information</td>
 					<td colspan="2">Number of amino acids</td>
-					<td> 241</td>
+					<td> 514</td>
 				</tr>
 				<tr>
 					<td colspan="2">Molecular weight</td>
-					<td> 25770.01</td>
+					<td> 56797.79</td>
 				</tr>
 				<tr>
 					<td colspan="2">Theoretical pi</td>
-					<td> 5.14</td>
+					<td> 4.96</td>
 				</tr>
 				<tr>
 					<td colspan="2">Extinction coefficient</td>
-					<td> 21430</td>
+					<td> 55030 of 54780</td>
 				</tr>
 				<tr>
 					<td colspan="2">Formula</td>
-					<td> C<sub>1140</sub>H<sub>1807</sub>N<sub>313</sub>O<sub>359</sub>S<sub>4</sub></td>
+					<td> C<sub>2480</sub>H<sub>3950</sub>N<sub>680</sub>O<sub>808</sub>S<sub>18</sub></td>
 				</tr>
 				<tr>
 					<td colspan="2">Total numbers of atons</td>
-					<td> 3623</td>
+					<td> 7936 </td>
 				</tr>
 				<tr>
-					<td style="font-weight:bold; text-align:center;text-decoration: " rowspan="40">Amino Acid Composition</td>
+					<td style="font-weight:bold;" rowspan="40">Amino Acid Composition</td>
-					<td style="font-weight:bold;text-align:center;text-decoration: underline;">Amino Acid</td>
+					<td style="font-weight:bold;">Amino Acid</td>
-					<td style="font-weight:bold;text-align:center;text-decoration: underline;">Frequency</td>
+					<td style="font-weight:bold;">Frequency</td>
-					<td style="font-weight:bold;text-align:center;text-decoration: underline;">Percentage(%)</td>
+					<td style="font-weight:bold;">Percentage(%)</td>
 				</tr>
 				<tr>
 					<td>Ala(A)</td>
-					<td>11</td>
+					<td>53</td>
-					<td>4.6</td>
+					<td>10.3</td>
 				</tr>
 				<tr>
 					<td>Arg(R</td>
-					<td>9</td>
+					<td>28</td>
-					<td>3.7</td>
+					<td>5.4</td>
 				</tr>
 				<tr>
 					<td>Asn(N)</td>
-					<td>11</td>
+					<td>22</td>
-					<td>4.6</td>
+					<td>4.3</td>
 				</tr>
 				<tr>
 					<td>Asp(D)</td>
-					<td>12</td>
+					<td>22</td>
-					<td>5.0</td>
+					<td>4.3</td>
 				</tr>
 				<tr>
 					<td>Cys(C)</td>
-					<td>0</td>
+					<td>4</td>
-					<td>0</td>
+					<td>0.8</td>
 				</tr>
 				<tr>
 					<td>Gln(Q)</td>
-					<td>11</td>
+					<td>12</td>
-					<td>4.6</td>
+					<td>2.3</td>
 				</tr>
 				<tr>
 					<td>Glu(E)</td>
-					<td>16</td>
+					<td>60</td>
-					<td>6.6</td>
+					<td>11.7</td>
 				</tr>
 				<tr>
 					<td>Gly(G)</td>
-					<td>35</td>
+					<td>39</td>
-					<td>14.5</td>
+					<td>7.6</td>
 				</tr>
 				<tr>
 					<td>His(H)</td>
-					<td>5</td>
+					<td>6</td>
-					<td>2.1</td>
+					<td>1.2</td>
 				</tr>
 				<tr>
 					<td>Ile(I)</td>
-					<td>19</td>
+					<td>28</td>
-					<td>7.9</td>
+					<td>5.4</td>
 				</tr>
 				<tr>
 					<td>Leu(L)</td>
-					<td>20</td>
+					<td>53</td>
-					<td>8.3</td>
+					<td>10.3</td>
 				</tr>
 				<tr>
 					<td>Lys(K)</td>
-					<td>11</td>
+					<td>32</td>
-					<td>4.6</td>
+					<td>6.2</td>
 				</tr>
 				<tr>
 					<td>Met(M)</td>
-					<td>4</td>
+					<td>14</td>
-					<td>1.7</td>
+					<td>2.7</td>
 				</tr>
 				<tr>
 					<td>Phe(F)</td>
-					<td>8</td>
+					<td>12</td>
-					<td>3.3</td>
+					<td>2.3</td>
 				</tr>
 				<tr>
 					<td>Pro(P)</td>
-					<td>7</td>
+					<td>10</td>
-					<td>2.9</td>
+					<td>1.9</td>
 				</tr>
 				<tr>
 					<td>Ser(S)</td>
-					<td>19</td>
+					<td>49</td>
-					<td>7.9</td>
+					<td>9.5</td>
 				</tr>
 				<tr>
 					<td>Thr(T)</td>
-					<td>13</td>
+					<td>24</td>
-					<td>5.4</td>
+					<td>4.7</td>
 				</tr>
 				<tr>
 					<td>Trp(W)</td>
-					<td>2</td>
+					<td>4</td>
 					<td>0.8</td>
 				</tr>
 				<tr>
 					<td>Tyr(T)</td>
-					<td>7</td>
+					<td>22</td>
-					<td>2.9</td>
+					<td>4.3</td>
 				</tr>
 				<tr>
 					<td>Val(V)</td>
-					<td>21</td>
+					<td>20</td>
-					<td>8.7</td>
+					<td>3.9</td>
 				</tr>
 				<tr>
@@ Line 183: / Line 183: @@
 				</tr>
 			</table>