Difference between revisions of "Part:BBa K1992011:Design"

Latest revision as of 21:28, 15 October 2016

Histamin-Tar expression system(promoter+RBS+coding+terminator)

Novel Histamine-Tar chemoreceptor in E.coli

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1355
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 184

Design Notes

Primers used for inserting point mutations at region I:
Forward: 5'-TCA GCG GTA CGG ATG ATG ATG GA- 3'
Reverse: 5'-ACG CCA CAG GTT AAT TTC CGT TTG CAG CAT- 3'
Primers used for inserting point mutations region II:
Forward: 5'-ACC TGG GGA ATG ATG AAT GCA ATG GGC- 3'
Reverse: 5'-TGG ATG AGC CAC TTC AGC TCC AGT ATT- 3'

@@ Line 1: / Line 1: @@
 __NOTOC__
+<!-- ######################## TITLE ######################### -->
 <partinfo>BBa_K1992011 short</partinfo>
+<!-- ######################## SUB TITLE ######################### -->
+Novel Histamine-Tar chemoreceptor in E.coli
+<!-- ######################## SEQUENCE ######################### -->
+<span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K1992011 SequenceAndFeatures</partinfo>
 ===Design Notes===
-Enter  mutations...
+Primers used for inserting point mutations at region I:<br>
+Forward: 5'-TCA GCG GTA CGG ATG ATG ATG GA- 3'<br>
+Reverse: 5'-ACG CCA CAG GTT AAT TTC CGT TTG CAG CAT- 3'<br>
+Primers used for inserting point mutations region II:<br>
-===Source===
+Forward: 5'-ACC TGG GGA ATG ATG AAT GCA ATG GGC- 3'<br>
+Reverse: 5'-TGG ATG AGC CAC TTC AGC TCC AGT ATT- 3'<br>
-Rosetta, E-coli....
-===References===