Difference between revisions of "Part:BBa K2042011:Design"
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===Source=== | ===Source=== | ||
| − | The RBS were synthesized from the genomic sequence of P.putida | + | The RBS were synthesized from the genomic sequence of P.putida and the PhaC gene from Pseudomonas resinovorans. |
===References=== | ===References=== | ||
Latest revision as of 21:22, 15 October 2016
RBS +PhaC_resinovorans
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 100
Illegal XhoI site found at 185
Illegal XhoI site found at 1043 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1073
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 809
Design Notes
We inserted non-coding scar sequences after RBS witch is close to the gene to prevent missing initial codons during translation.
Source
The RBS were synthesized from the genomic sequence of P.putida and the PhaC gene from Pseudomonas resinovorans.