Difference between revisions of "Part:BBa K546000:Experience"

Revision as of 21:21, 15 October 2016

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Applications of BBa_K546000

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This part was tested in conjunction with BBa_K546005

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<I>tmswartjes

We tested BBa_K546000 in pSB4K5 with a HSL reporter (BBa_K1913014) in pSB1C3. E. coli DH5alpha cells were transformed with both plasmids (cells containing BBa_K546000 were used to make elctrocompetent cells, which were then transformed with the reporter), these cells were tested overnight in BioTek’s SynergyMx plate reader overnight.

Fluorescence and absorbance data for E. coli quorum sensing strains. Error bars indicate standard error of the mean. Continuous line: Fluorescence/OD600; Dashed line: Absorbance at OD600. Orange, Blue: separate biological repeats of the DH5alpha strains containing the 2 plasmids (BBa_K546000 in pSB4K5 and BBa_K1913014 in pSB1C3). Pink: reporter plasmid only strain. For both strains every value displayed is the average of all (4) technical replicates. All Fluorescence/OD600 data were normalized to the highest Fluorescence/OD600 value measured. After almost 12 hours, the fluorescence from the 2 plasmid strains reached an overflow. Therefore no values after this point are displayed. One biological repeat of the 2 plasmid strain experiment is not shown to improve the clarity of the graph. For completeness, the complete graph is shown underneath here.

Fluorescence and absorbance data for E. coli quorum sensing strains. Error bars indicate standard error of the mean. Continuous line: Fluorescence/OD600; Dashed line: Absorbance at OD600. Orange, Blue, Green: separate biological repeats of the DH5alpha strains containing the 2 plasmids (BBa_K546000 in pSB4K5 and BBa_K1913014 in pSB1C3). Pink: reporter plasmid only strain. For both strains every value displayed is the average of all (4) technical replicates. All Fluorescence/OD600 data were normalized to the highest Fluorescence/OD600 value measured. After almost 12 hours, the fluorescence from the 2 plasmid strains reached an overflow. Therefore no values after this point are displayed. One biological repeat (green) showed a high variance between technical repeats in both OD600 and Fluorescence/OD600 values.

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UNIQ8537163d0544ea48-partinfo-00000003-QINU

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 <p>
-We tested BBa_K546000 in pSB4K5 with a HSL reporter (BBa_K1913014) in pSB1C3. <i>E. coli</i> DH5alpha cells were transformed with both plasmids (cells containing BBa_K546000 were used to make elctrocompetent cells, which were then transformed with the reporter), these cells were tested overnight in BioTek’s SynergyMx plate reader overnight. </p>
+We tested BBa_K546000 in pSB4K5 with a HSL reporter (BBa_K1913014) in pSB1C3. <i>E. coli</i> DH5alpha cells were transformed with both plasmids (cells containing BBa_K546000 were used to make elctrocompetent cells, which were then transformed with the reporter), these cells were tested overnight in BioTek’s SynergyMx plate reader overnight. </p><br><br>
 https://static.igem.org/mediawiki/2016/a/a1/T--Wageningen_UR--2plasmid_quorum_sensing_graph.jpg
-<p> Fluorescence and absorbance data for E. coli quorum sensing strains. Error bars indicate standard error of the mean. Continuous line: Fluorescence/OD600; Dashed line: Absorbance at OD600. Orange, Blue: separate biological repeats of the DH5alpha strains containing the 2 plasmids (BBa_K546000 in pSB4K5 and  BBa_K1913014 in pSB1C3). Pink: reporter plasmid only strain. For both strains every value displayed is the average of all (4) technical replicates. All Fluorescence/OD600 data were normalized to the highest Fluorescence/OD600 value measured. After almost 12 hours, the fluorescence from the 2 plasmid strains reached an overflow. Therefore no values after this point are displayed. One biological repeat of the 2 plasmid strain experiment is not shown to improve the clarity of the graph. For completeness, the complete graph is shown underneath here. </p>
+<p> Fluorescence and absorbance data for E. coli quorum sensing strains. Error bars indicate standard error of the mean. Continuous line: Fluorescence/OD600; Dashed line: Absorbance at OD600. Orange, Blue: separate biological repeats of the DH5alpha strains containing the 2 plasmids (BBa_K546000 in pSB4K5 and  BBa_K1913014 in pSB1C3). Pink: reporter plasmid only strain. For both strains every value displayed is the average of all (4) technical replicates. All Fluorescence/OD600 data were normalized to the highest Fluorescence/OD600 value measured. After almost 12 hours, the fluorescence from the 2 plasmid strains reached an overflow. Therefore no values after this point are displayed. One biological repeat of the 2 plasmid strain experiment is not shown to improve the clarity of the graph. For completeness, the complete graph is shown underneath here. </p><br><br>
 https://static.igem.org/mediawiki/2016/9/9c/T--Wageningen_UR--complete_QS_2plasmid_graph.jpg