Difference between revisions of "Part:BBa K2042011:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | We inserted non-coding scar sequences after RBS witch is close to the gene to prevent missing initial codons during translation. | |
===Source=== | ===Source=== | ||
Revision as of 21:19, 15 October 2016
RBS +PhaC_resinovorans
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 100
Illegal XhoI site found at 185
Illegal XhoI site found at 1043 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1073
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 809
Design Notes
We inserted non-coding scar sequences after RBS witch is close to the gene to prevent missing initial codons during translation.
Source
Synthetic synthesis