Difference between revisions of "Part:BBa K2042010:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | We inserted non-coding scar sequences after RBS witch is close to the gene to prevent missing initial codons during translation. | |
===Source=== | ===Source=== | ||
Latest revision as of 21:19, 15 October 2016
RBS + Pct
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 688
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1094
Illegal BsaI site found at 1565
Design Notes
We inserted non-coding scar sequences after RBS witch is close to the gene to prevent missing initial codons during translation.
Source
The RBS were synthesized from the genomic sequence of P.putida, Pct gene were synthetised from genomic sequence of Clostridum propionicum.