Difference between revisions of "Part:BBa K1992002:Design"
 
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===Design Notes===
 
===Design Notes===
−
Primers used for inserting point mutations at the first region:<br>
+
Primers used for inserting point mutations at region I:<br>
 
Forward: 5'-TCA GCG GTA CGG ATG ATG ATG GA- 3'<br>
 
Forward: 5'-TCA GCG GTA CGG ATG ATG ATG GA- 3'<br>
 
Reverse: 5'-ACG CCA CAG GTT AAT TTC CGT TTG CAG CAT- 3'<br>
 
Reverse: 5'-ACG CCA CAG GTT AAT TTC CGT TTG CAG CAT- 3'<br>
−
Primers used for inserting point mutations at the second region:<br>
+
Primers used for inserting point mutations region II:<br>
 
Forward: 5'-ACC TGG GGA ATG ATG AAT GCA ATG GGC- 3'<br>
 
Forward: 5'-ACC TGG GGA ATG ATG AAT GCA ATG GGC- 3'<br>
 
Reverse: 5'-TGG ATG AGC CAC TTC AGC TCC AGT ATT- 3'<br>
 
Reverse: 5'-TGG ATG AGC CAC TTC AGC TCC AGT ATT- 3'<br>

Latest revision as of 21:15, 15 October 2016

Histamin-Tar receptor

Novel Histamine-Tar chemoreceptor in E.coli

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1282
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 111


Design Notes

Primers used for inserting point mutations at region I:
Forward: 5'-TCA GCG GTA CGG ATG ATG ATG GA- 3'
Reverse: 5'-ACG CCA CAG GTT AAT TTC CGT TTG CAG CAT- 3'
Primers used for inserting point mutations region II:
Forward: 5'-ACC TGG GGA ATG ATG AAT GCA ATG GGC- 3'
Reverse: 5'-TGG ATG AGC CAC TTC AGC TCC AGT ATT- 3'