Difference between revisions of "Part:BBa K1992002:Design"
Line 12: Line 12:
  
 
===Design Notes===
 
===Design Notes===
−
Primers used for inserting point mutations at the first region:
+
Primers used for inserting point mutations at the first region:</b>
−
Forward: 5'-TCA GCG GTA CGG ATG ATG ATG GA- 3'
+
Forward: 5'-TCA GCG GTA CGG ATG ATG ATG GA- 3'</b>
 
Reverse: 5'-ACG CCA CAG GTT AAT TTC CGT TTG CAG CAT- 3'
 
Reverse: 5'-ACG CCA CAG GTT AAT TTC CGT TTG CAG CAT- 3'
 
Primers used for inserting point mutations at the second region:
 
Primers used for inserting point mutations at the second region:
 
Forward: 5'-ACC TGG GGA ATG ATG AAT GCA ATG GGC- 3'
 
Forward: 5'-ACC TGG GGA ATG ATG AAT GCA ATG GGC- 3'
 
Reverse: 5'-TGG ATG AGC CAC TTC AGC TCC AGT ATT- 3'
 
Reverse: 5'-TGG ATG AGC CAC TTC AGC TCC AGT ATT- 3'

Revision as of 21:13, 15 October 2016

Histamin-Tar receptor

Novel Histamine-Tar chemoreceptor in E.coli

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1282
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 111


Design Notes

Primers used for inserting point mutations at the first region:</b> Forward: 5'-TCA GCG GTA CGG ATG ATG ATG GA- 3'</b> Reverse: 5'-ACG CCA CAG GTT AAT TTC CGT TTG CAG CAT- 3' Primers used for inserting point mutations at the second region: Forward: 5'-ACC TGG GGA ATG ATG AAT GCA ATG GGC- 3' Reverse: 5'-TGG ATG AGC CAC TTC AGC TCC AGT ATT- 3'