Difference between revisions of "Part:BBa K1886019:Design"

(Design Notes)
(Source)
 
Line 11: Line 11:
 
===Source===
 
===Source===
  
xxxxxxxxxxxxxxxxxxxxx
+
The subparts are from E coli genome, obtained from iGEM team HZAU's lab.
  
 
===References===
 
===References===

Latest revision as of 19:11, 15 October 2016


pSB1C3_Ho1_pcyA_Pompc_CI_GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1607
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1160
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2899


Design Notes

The existence of double terminator can make it difficult for pcr or overlap pcr.

Source

The subparts are from E coli genome, obtained from iGEM team HZAU's lab.

References