Difference between revisions of "Part:BBa K1886019:Design"
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===Source=== | ===Source=== | ||
− | + | The subparts are from E coli genome, obtained from iGEM team HZAU's lab. | |
===References=== | ===References=== |
Latest revision as of 19:11, 15 October 2016
pSB1C3_Ho1_pcyA_Pompc_CI_GFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1607
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1160
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 2899
Design Notes
The existence of double terminator can make it difficult for pcr or overlap pcr.
Source
The subparts are from E coli genome, obtained from iGEM team HZAU's lab.