Difference between revisions of "Part:BBa K1995014"
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<partinfo>BBa_K1995025 short</partinfo> | <partinfo>BBa_K1995025 short</partinfo> | ||
Based on BBa_K1420001 (merA), we added INP-N to the upstream of merA. Using INP-N, we anchored merA out of the outer membrane of E.coli in order to increase the reducing efficiency of mercuric ions. | Based on BBa_K1420001 (merA), we added INP-N to the upstream of merA. Using INP-N, we anchored merA out of the outer membrane of E.coli in order to increase the reducing efficiency of mercuric ions. | ||
| − | + | ==Ice nucleation protein (INP)== | |
To increase the reducing efficiency and detect the concentration of heavy metals, we used INP to anchor merA out of the outer membrane of E. coli. | To increase the reducing efficiency and detect the concentration of heavy metals, we used INP to anchor merA out of the outer membrane of E. coli. | ||
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[[File:INP.png]] | [[File:INP.png]] | ||
| − | + | ==Binding heavy metals== | |
We detect the reducing efficiency of IA and other parts and get the following chart. (Fig. 2) | We detect the reducing efficiency of IA and other parts and get the following chart. (Fig. 2) | ||
[[File:0142.png]] | [[File:0142.png]] | ||
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Latest revision as of 18:48, 15 October 2016
this part can bind heavy metal ions
Based on BBa_K1420001 (merA), we added INP-N to the upstream of merA. Using INP-N, we anchored merA out of the outer membrane of E.coli in order to increase the reducing efficiency of mercuric ions.
Ice nucleation protein (INP)
To increase the reducing efficiency and detect the concentration of heavy metals, we used INP to anchor merA out of the outer membrane of E. coli.
Ice nucleation protein (INP) is an excretion surface protein. It is widely used for establishing germ surface display system. It contains an internal repeated domain (IRD) and an N-terminal anchoring domain (fig.1). Studies have shown that decrease the time of repetition of IRD can also get a better-off rate of displaying. So we decreased the time of repetition of IRD to reduce pressure of synthesis of E. coli.
Binding heavy metals
We detect the reducing efficiency of IA and other parts and get the following chart. (Fig. 2)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]