Difference between revisions of "Part:BBa K1995007"
 
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__NOTOC__
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__TOC__
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<partinfo>BBa_K1995007 short</partinfo>
 
<partinfo>BBa_K1995007 short</partinfo>
  
 
Based on BBa_K1995004 (BC), we added INP-N to the upstream of BC. Using INP-N, we anchored BC out of the outer membrane of E.coli in order to increase the binding efficiency of cupric and mercuric ions.
 
Based on BBa_K1995004 (BC), we added INP-N to the upstream of BC. Using INP-N, we anchored BC out of the outer membrane of E.coli in order to increase the binding efficiency of cupric and mercuric ions.
  
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'''Ice nucleation protein (INP)'''
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==Ice nucleation protein (INP)==
  
 
To increase the binding efficiency and detect the concentration of heavy metals, we used INP to anchor BC out of the outer membrane of E. coli.
 
To increase the binding efficiency and detect the concentration of heavy metals, we used INP to anchor BC out of the outer membrane of E. coli.
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[[File:INP.png]]
 
[[File:INP.png]]
  
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'''Binding heavy metals'''
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==Binding heavy metals==
  
 
We detect the binding efficiency of PC and other parts and get the following chart. (Fig. 2)
 
We detect the binding efficiency of PC and other parts and get the following chart. (Fig. 2)
 
   
 
   
 
[[File:0072.png]]
 
[[File:0072.png]]
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 18:35, 15 October 2016


PC (INP-N-C)

Based on BBa_K1995004 (BC), we added INP-N to the upstream of BC. Using INP-N, we anchored BC out of the outer membrane of E.coli in order to increase the binding efficiency of cupric and mercuric ions.

Ice nucleation protein (INP)

To increase the binding efficiency and detect the concentration of heavy metals, we used INP to anchor BC out of the outer membrane of E. coli.

Ice nucleation protein (INP) is an excretion surface protein. It is widely used for establishing germ surface display system. It contains an internal repeated domain (IRD) and an N-terminal anchoring domain (fig.1). Studies have shown that decrease the time of repetition of IRD can also get a better-off rate of displaying. So we decreased the time of repetition of IRD to reduce pressure of synthesis of E. coli.

INP.png

Binding heavy metals

We detect the binding efficiency of PC and other parts and get the following chart. (Fig. 2)

0072.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 429
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 238