Difference between revisions of "Part:BBa I712009"
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This part was submitted before the RFC[25] standard was established. Thus this part carries a modified RFC[10] pre- and suffix that changes the reading frame of the suffix in a way that, if you ligate it to a second part, it does not generate a stop codon. Although this approach makes it possible to produce fusion proteins the ligation to a second part, which does not carry the modified RFC[10] leads to a frameshift in the second part.
 
This part was submitted before the RFC[25] standard was established. Thus this part carries a modified RFC[10] pre- and suffix that changes the reading frame of the suffix in a way that, if you ligate it to a second part, it does not generate a stop codon. Although this approach makes it possible to produce fusion proteins the ligation to a second part, which does not carry the modified RFC[10] leads to a frameshift in the second part.
  
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Because signal peptides for eucaryotic cells are of importance we designed new signal peptide parts such as [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2170215 BBa_K2170215] to help other iGEM teams to get a working part.
+
Because signal peptides for eucaryotic cells are of importance we designed new signal peptide parts such as [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2170215 BBa_K2170215] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2170214 BBa_K2170214] to help other iGEM teams to get a working part.
  
  

Revision as of 17:48, 15 October 2016


CD4 signal peptide; localizes to plasma membrane


Additional characterization of BBaI712009 by [http://2016.igem.org/Team:LMU-TUM_Munich Munich 2016]:

This part was submitted before the RFC[25] standard was established. Thus this part carries a modified RFC[10] pre- and suffix that changes the reading frame of the suffix in a way that, if you ligate it to a second part, it does not generate a stop codon. Although this approach makes it possible to produce fusion proteins the ligation to a second part, which does not carry the modified RFC[10] leads to a frameshift in the second part.

Because signal peptides for eucaryotic cells are of importance we designed new signal peptide parts such as BBa_K2170215 and BBa_K2170214 to help other iGEM teams to get a working part.



Coding region for first 25 amino acids (including start codon) of CD4 receptor which is signal peptide for plasma membrane localization.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]