Difference between revisions of "Part:BBa I712009"

Revision as of 17:45, 15 October 2016

CD4 signal peptide; localizes to plasma membrane

Additional characterization of BBaI712009 by [http://2016.igem.org/Team:LMU-TUM_Munich Munich 2016]:

This part was submitted before the RFC[25] standard was established. Thus this part carries a modified RFC[10] pre- and suffix that changes the reading frame of the suffix in a way that, if you ligate it to a second part, it does not generate a stop codon. Although this approach makes it possible to produce fusion proteins the ligation to a second part, which does not carry the modified RFC[10] leads to a frameshift in the second part.

This is why we designed new signal peptide parts such as BBa_K2170215

Coding region for first 25 amino acids (including start codon) of CD4 receptor which is signal peptide for plasma membrane localization.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_I712009 short</partinfo>
+Additional characterization of BBaI712009 by [http://2016.igem.org/Team:LMU-TUM_Munich Munich 2016]:
+This part was submitted before the RFC[25] standard was established. Thus this part carries a modified RFC[10] pre- and suffix that changes the reading frame of the suffix in a way that, if you ligate it to a second part, it does not generate a stop codon. Although this approach makes it possible to produce fusion proteins the ligation to a second part, which does not carry the modified RFC[10] leads to a frameshift in the second part.
+This is why we designed new signal peptide parts such as [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2170215 BBa_K2170215]
 Coding region for first 25 amino acids (including start codon) of CD4 receptor which is signal peptide for plasma membrane localization.
@@ Line 11: / Line 17: @@
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_I712009 SequenceAndFeatures</partinfo>
-Additional characterization of BBaI712009 by [http://2016.igem.org/Team:LMU-TUM_Munich Munich 2016]:
-This part was submitted before the RFC[25] standard was established. Thus this part carries a modified RFC[10] pre- and suffix that changes the reading frame of the suffix in a way that, if you ligate it to a second part, it does not generate a stop codon. Although this approach makes it possible to produce fusion proteins the ligation to a second part, which does not carry the modified RFC[10] leads to a frameshift in the second part.
-This is why we designed new signal peptide parts such as [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2170215 BBa_K2170215]