Difference between revisions of "Part:BBa K1897015:Design"

(→‎Design Notes)
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===References===
 
===References===
 +
Choi, S. H., & Greenberg, E. P. (1992). Genetic evidence for multimerization of LuxR, the transcriptional activator of Vibrio fischeri luminescence. <i>Molecular marine biology and biotechnology, 1</i>(6), 408-413.
 +
 +
Fuqua, W. C., Winans, S. C., & Greenberg, E. P. (1994). Quorum sensing in bacteria: the LuxR-LuxI family of cell density-responsive transcriptional regulators. <i>Journal of bacteriology, 176</i>(2), 269.
 +
 +
Shadel, G. S., & Baldwin, T. O. (1991). The Vibrio fischeri LuxR protein is capable of bidirectional stimulation of transcription and both positive and negative regulation of the luxR gene. <i>Journal of bacteriology, 173</i>(2), 568-574.
 +
 +
Trott, A. E. (2000). Amino Acid Residues in LuxR Critical for its Mechanism of Transcriptional Activation during Quorum Sensing (Doctoral dissertation, Virginia Polytechnic Institute and State University).

Revision as of 17:43, 15 October 2016


LuxR + LuxI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1852
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 50
    Illegal AgeI site found at 1065
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is one HA tag available for characterisation of the LuxR and LuxI proteins respectively produced via western blot.

Source

These parts are derived from Vibrio fischeri.

References

Choi, S. H., & Greenberg, E. P. (1992). Genetic evidence for multimerization of LuxR, the transcriptional activator of Vibrio fischeri luminescence. Molecular marine biology and biotechnology, 1(6), 408-413.

Fuqua, W. C., Winans, S. C., & Greenberg, E. P. (1994). Quorum sensing in bacteria: the LuxR-LuxI family of cell density-responsive transcriptional regulators. Journal of bacteriology, 176(2), 269.

Shadel, G. S., & Baldwin, T. O. (1991). The Vibrio fischeri LuxR protein is capable of bidirectional stimulation of transcription and both positive and negative regulation of the luxR gene. Journal of bacteriology, 173(2), 568-574.

Trott, A. E. (2000). Amino Acid Residues in LuxR Critical for its Mechanism of Transcriptional Activation during Quorum Sensing (Doctoral dissertation, Virginia Polytechnic Institute and State University).