Difference between revisions of "Part:BBa K1993001"
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<partinfo>BBa_K1993001 parameters</partinfo>
 
<partinfo>BBa_K1993001 parameters</partinfo>
 
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<h1 id="toc_0">Design</h1>
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<h2 id="toc_1">Background</h2>
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 +
<p><em>Inflammmation</em>  During the inlflammation process of multiple diseases,such as IBD and artritis,chemoattraction plays a pivotal role,taking the advantae of chemokines and cytokine in target to its correspongding receptors.<br>
 +
<em>Chemotaxis</em>  Therefore,stem-cell researchers attempt to transport MSC, a kind of mesenchymal stem cell with outstanding capability of inducing issue regenereation and anti-infllammation,to the injured position for cell therapy.Genetically engineered MSCs with upregulated chemokine receptor can obtain more efficient chemoatrraction ability.</p>
 +
 +
<h2 id="toc_2">Subparts</h2>
 +
 +
<h3 id="toc_3">Promotor</h3>
 +
 +
<p>Chemokine receptors are expressed under the control of constructive promoter EF-1α therefore they could be consistently expressed by MSCs.EF1-α induces the prolonged transcription from plasimid DNA to RNA in Human MSCs,and then the RNA will be translated into proteins and expresses in MSCs, like chemokine receptors are integrated onto the celluar membrane.</p>
 +
 +
<h3 id="toc_4">Chemokine Receptor</h3>
 +
 +
<p>Chemokine receptors are cytokine receptors found on the surface of certain cells that interact with a type of cytokine called a chemokine. They have a 7-transmembrane (7TM) structure and couples to G-protein for signal transduction within a cell [^emphasize] (CXCR5)(Figure 1). Following interaction with their specific chemokine ligands, chemokine receptors trigger a flux inintracellular calcium (Ca2+) ions which initiate the onset of chemotaxis that traffics the cell to a desired location (Figure 2).  </p>
 +
 +
<ul>
 +
<li><p><strong>CXCR4</strong></p>
 +
 +
<ul>
 +
<li><p><em>disease</em></p></li>
 +
<li><p><em>function</em></p></li>
 +
</ul></li>
 +
<li><p><strong>CXCR5</strong></p>
 +
 +
<ul>
 +
<li><p><em>disease</em></p></li>
 +
<li><p><em>function</em></p></li>
 +
</ul></li>
 +
</ul>
 +
 +
<h3 id="toc_5">Marking protein</h3>
 +
 +
<ul>
 +
<li><strong>eFTH</strong></li>
 +
<li><strong>eGFP</strong></li>
 +
<li><strong>Luciferase</strong></li>
 +
<li><strong>dTomato</strong></li>
 +
</ul>
 +
 +
<h3 id="toc_6">IRES</h3>
 +
 +
<h3 id="toc_7">T2A</h3>
 +
 +
<h3 id="toc_8">attB1/attB2</h3>
 +
 +
<ul>
 +
<li><strong>attB1</strong><br></li>
 +
<li><strong>attB2</strong></li>
 +
</ul>
 +
 +
<h2 id="toc_9">Composite parts</h2>
 +
 +
<h3 id="toc_10">Protein coding sequences</h3>
 +
 +
<ul>
 +
<li>CCR7<br></li>
 +
<li>CXCR1<br></li>
 +
<li>CXCR4<br></li>
 +
<li>CCR5<br></li>
 +
</ul>
 +
 +
<h3 id="toc_11">Composite parts</h3>
 +
 +
<p>Luciferase-IRES-eGFP<br>
 +
Luciferase-T2A-dtomato-T2A-hFTH<br>
 +
CXCR4-IRES-eGFP<br>
 +
CXCR5-IRES-eGFP<br>
 +
CXCR4-T2A-Luciferase-IRES-eGFP  </p>
 +
 +
 +
 +
 +
</body>
 +
 +
</html>

Revision as of 16:20, 15 October 2016


CCR7


Chemokine receptors are found on the surface of certain cells that interact with chemokines. They have a 7-transmembrane (7TM) structure and couples to G-protein for signal transduction within a cell[1] (Figure 1). Following interaction with their specific chemokine ligands, chemokine receptors trigger a flux inintracellular calcium (Ca2+) ions which initiate the onset of chemotaxis that traffics the cell to a desired location (Figure 2).


Figure 1. typical structure of a chemokine receptor.

References

[1]Allen, Samantha J.; Crown, Susan E.; Handel, Tracy M. (2007-01-01). "Chemokine: receptor structure, interactions, and antagonism". Annual Review of Immunology. 25: 787–820.

[2] Griffith J W, Sokol C L, Luster A D. Chemokines and chemokine receptors: positioning cells for host defense and immunity.[J]. Annual Review of Immunology, 2014, 32(1):659-702.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1111
    Illegal SapI site found at 1017



<!DOCTYPE html> description

Design

Background

Inflammmation During the inlflammation process of multiple diseases,such as IBD and artritis,chemoattraction plays a pivotal role,taking the advantae of chemokines and cytokine in target to its correspongding receptors.
Chemotaxis Therefore,stem-cell researchers attempt to transport MSC, a kind of mesenchymal stem cell with outstanding capability of inducing issue regenereation and anti-infllammation,to the injured position for cell therapy.Genetically engineered MSCs with upregulated chemokine receptor can obtain more efficient chemoatrraction ability.

Subparts

Promotor

Chemokine receptors are expressed under the control of constructive promoter EF-1α therefore they could be consistently expressed by MSCs.EF1-α induces the prolonged transcription from plasimid DNA to RNA in Human MSCs,and then the RNA will be translated into proteins and expresses in MSCs, like chemokine receptors are integrated onto the celluar membrane.

Chemokine Receptor

Chemokine receptors are cytokine receptors found on the surface of certain cells that interact with a type of cytokine called a chemokine. They have a 7-transmembrane (7TM) structure and couples to G-protein for signal transduction within a cell [^emphasize] (CXCR5)(Figure 1). Following interaction with their specific chemokine ligands, chemokine receptors trigger a flux inintracellular calcium (Ca2+) ions which initiate the onset of chemotaxis that traffics the cell to a desired location (Figure 2).

  • CXCR4

    • disease

    • function

  • CXCR5

    • disease

    • function

Marking protein

  • eFTH
  • eGFP
  • Luciferase
  • dTomato

IRES

T2A

attB1/attB2

  • attB1
  • attB2

Composite parts

Protein coding sequences

  • CCR7
  • CXCR1
  • CXCR4
  • CCR5

Composite parts

Luciferase-IRES-eGFP
Luciferase-T2A-dtomato-T2A-hFTH
CXCR4-IRES-eGFP
CXCR5-IRES-eGFP
CXCR4-T2A-Luciferase-IRES-eGFP