Difference between revisions of "Part:BBa K1830002:Experience"
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iGEMers who want to use the SPusp45, they should choose the BBa K1654007 part, we have verified that BBa K1654007 is functional in protein secretion. | iGEMers who want to use the SPusp45, they should choose the BBa K1654007 part, we have verified that BBa K1654007 is functional in protein secretion. | ||
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===Author=== | ===Author=== | ||
Yifei Lu | Yifei Lu | ||
Revision as of 14:45, 15 October 2016
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1830002
BBa K1830002 is stated as the signal peptide of Usp45, however,after thorough literature search we found that SPusp45 is only 27 amino acids long, but not the current version of BBa_K1830002. We confirmed that the 27 amino acids long SPusp45 signal peptide can be used for secreting proteins out of the L.lactis cell. It should be fused to the N-terminal or C-terminal of the protein of interest.
iGEMers who want to use the SPusp45, they should choose the BBa K1654007 part, we have verified that BBa K1654007 is functional in protein secretion.
Author
Yifei Lu
Summary
We characterized that the 27 amino acids long SPusp45 signal peptide part BBa K1654007, but not the BBa_K1830002 part is the right choice that can be used to secret proteins out of L. lactis.
User Reviews
UNIQe5d7483fc79815a0-partinfo-00000000-QINU
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TMMU-China-2016 |
BBa K1830002 is stated as the signal peptide of Usp45, however,after thorough literature search we found that SPusp45 is only 27 amino acids long, but not the current version of BBa_K1830002. We confirmed that the 27 amino acids long SPusp45 signal peptide can be used for secreting proteins out of the L.lactis cell. It should be fused to the N-terminal or C-terminal of the protein of interest. iGEMers who want to use the SPusp45, they should choose the BBa K1654007 part, we have verified that BBa K1654007 is functional in protein secretion. |
UNIQe5d7483fc79815a0-partinfo-00000002-QINU