Difference between revisions of "Part:BBa K2060001"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | We attempted to use arabinose to induce expression of this biobrick variety of concentrations and time periods. Our success with this expression are documented in the Experience section. | + | We attempted to use arabinose to induce expression of this biobrick variety of concentrations and time periods. Our success with this expression are documented in the [https://parts.igem.org/Part:BBa_K2060001:Experience Experience] section. |
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Revision as of 14:39, 15 October 2016
Fluorescent protein mKeima expressed by Arabinose promotor
mKeima is a red-shifted fluorescent protein with Excitation of 440nM and Emission of 616nM. In this part mKeima expression is induced by Arabinose driven by the wildtype pBAD promotor. The red shifted emission spectrum gives it good potential for mammalian cell or tissue bioimaging.
Usage and Biology
We attempted to use arabinose to induce expression of this biobrick variety of concentrations and time periods. Our success with this expression are documented in the Experience section.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]