Difference between revisions of "Part:BBa K2012025"

 
Line 4: Line 4:
  
 
sfGFP Generator from pJT119b original plasmid.
 
sfGFP Generator from pJT119b original plasmid.
−
 
+
<html>
 +
<div>
 +
<div style="height:auto!important;margin-left:auto;margin-right:auto;min-height:841.9pt;padding-bottom:1pt;padding-top:1pt">
 +
<div style="height:auto!important;margin-left:auto;margin-right:auto;min-height:841.9pt;padding-bottom:1pt;padding-top:1pt">     
 +
<div style="margin-left:auto;margin-right:auto;position:relative">
 +
<div style="height:auto!important;margin-left:auto;margin-right:auto;min-height:841.9pt;padding-bottom:1pt;padding-top:1pt">     
 +
<div style="margin-left:auto;margin-right:auto;position:relative">
 +
<div style="height:auto!important;margin-left:auto;margin-right:auto;min-height:841.95pt;padding-bottom:1pt;padding-top:1pt">     
 +
<div style="margin-left:auto;margin-right:auto;position:relative">
 +
<div style="height:auto!important;margin-left:auto;margin-right:auto;min-height:841.9pt;padding-bottom:1pt;padding-top:1pt">     
 +
<div style="margin-left:auto;margin-right:auto;position:relative"><p style="text-align:justify;text-justify:inter-ideograph"><span style="font-family:Calibri"><img height="318.92999267578125" src="https://static.igem.org/mediawiki/2016/5/5f/Eeeee.jpeg" width="480.0"></span><span style="font-family:Calibri"><span></span></span></p>
 +
<p style="text-align:justify;text-justify:inter-ideograph"><span style="font-family:Calibri">&nbsp;</span></p>
 +
<p style="text-align:justify;text-justify:inter-ideograph"><span style="font-family:Calibri">Fluorescence assay of CcaS-CcaR system with PcpcG2-172 (BBa_K2012015) in CL1 (△EnvZ), and PCB (△CcaS) as </span><span style="font-family:Calibri">chromophore</span><span style="font-family:Calibri">. </span><span style="font-family:Calibri"><span></span></span></p>
 +
<p style="text-align:justify;text-justify:inter-ideograph"><span style="font-family:Calibri">F</span><span style="font-family:Calibri">luorescence under green light is 1.81-folds of red light, proving that green light activates output expression, the device works well. </span><span style="font-family:Calibri">PcpcG2-172 </span><span style="font-family:Calibri">shows high efficiency.<span></span></span></p>
 +
<p style="text-align:justify;text-justify:inter-ideograph"><span style="font-family:Calibri">More importantly, l</span><span style="font-family:Calibri">eaked expression in darkness significantly reduced after truncation of the constitutive promoter. </span><span style="font-family:Calibri;font-weight:bold"><span></span></span></p>
 +
<p style="text-align:justify;text-justify:inter-ideograph"><a name="_GoBack"></a><span style="font-family:Calibri">&nbsp;</span></p>
 +
</div>
 +
    </div>
 +
<p style="text-align:justify;text-justify:inter-ideograph"><br/>
 +
</p>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</html>
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 14:37, 15 October 2016


PcpcG2-172-B0034-sfGFP

sfGFP Generator from pJT119b original plasmid.

 

Fluorescence assay of CcaS-CcaR system with PcpcG2-172 (BBa_K2012015) in CL1 (△EnvZ), and PCB (△CcaS) as chromophore.

Fluorescence under green light is 1.81-folds of red light, proving that green light activates output expression, the device works well. PcpcG2-172 shows high efficiency.

More importantly, leaked expression in darkness significantly reduced after truncation of the constitutive promoter.

 


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]