Difference between revisions of "Part:BBa K2012023"

 
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===Usage and Biology===
 
===Usage and Biology===
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<div style="margin-left:auto;margin-right:auto;position:relative"><p style="text-align:justify;text-justify:inter-ideograph"><span style="font-family:Calibri">Promoter cpcG2 is a 238bp green-light activated promoter from the genome of Synechocystis PCC6803</span><span style="font-family:Calibri">. We tested the efficiency of the promoter by measuring the fluorescence of output sfGFP when bacteria are illuminated with green, red or no light. (For more detail, please view our wiki: </span><span style="font-family:Calibri">http://2016.igem.org/Team:HZAU-China/Experiments</span><span style="font-family:Calibri">)</span><br/>
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<p style="text-align:justify;text-justify:inter-ideograph"><span style="font-family:Calibri">&nbsp;</span></p>
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<p style="text-align:justify;text-justify:inter-ideograph"><span style="font-family:Calibri"><img height="293.1300048828125" src="https://static.igem.org/mediawiki/2016/7/7a/Pcpcg2.jpeg " width="480.0"></span><span style="font-family:Calibri"><span></span></span></p>
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<p style="text-align:justify;text-justify:inter-ideograph"><span style="font-family:Calibri">Fig.1 Mean sfGFP </span><span style="font-family:Calibri">fluorescence</span><span style="font-family:Calibri"> of CcaS-CcaR system under green light, red light and darkness.<span></span></span></p>
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<p style="text-align:justify;text-justify:inter-ideograph"><span style="font-family:Calibri">&nbsp;</span></p>
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<p style="text-align:justify;text-justify:inter-ideograph"><span style="font-family:Calibri">As is shown in the figure, in E.coli strain JT2, PcpcG2 </span><span style="font-family:Calibri">produces </span><span style="font-family:Calibri">266.0</span><span style="font-family:Calibri"> ± 1</span><span style="font-family:Calibri">9.</span><span style="font-family:Calibri">3 and </span><span style="font-family:Calibri">509.0</span><span style="font-family:Calibri"> ± </span><span style="font-family:Calibri">55.4</span><span style="font-family:Calibri"> au of</span><span style="font-family:Calibri"> </span><span style="font-family:Calibri">sfGFP in red and green light, corresponding to </span><span style="font-family:Calibri">1.91</span><span style="font-family:Calibri"> ± 0.3</span><span style="font-family:Calibri">4</span><span style="font-family:Calibri">-fold</span><span style="font-family:Calibri"> </span><span style="font-family:Calibri">activation</span><span style="font-family:Calibri">, demonstrating that PcpcG2 is functionally regulated by light, green-activated and red-repressed.<span></span></span></p>
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<p style="text-align:justify;text-justify:inter-ideograph"><span style="font-family:Calibri">&nbsp;</span></p>
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<p style="text-align:justify;text-justify:inter-ideograph"><span style="font-family:Calibri">However, the figure also shows that that leaked expression is severe in dark surroundings. To better respond to red and green light, we optimized the promoter by refactoring it and created an innovative promoter, PcpcG2-172, with the truncation of a constitutive promoter within the cpcG2 promoter </span><span style="font-family:Calibri">(</span><span style="font-family:Calibri">BBa_K</span><span style="font-family:Calibri">2012015</span><span style="font-family:Calibri">. </span><span style="font-family:Calibri">For more detail, please view our wiki: http://2016.igem.org/Team:HZAU-China/Experiments)</span><span style="font-family:Calibri">. </span><a name="_GoBack"></a><span style="font-family:Calibri"><span></span></span></p>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 14:24, 15 October 2016


PcpcG2-B0034-sfGFP

sfGFP Generator from pJT119b original plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]