Difference between revisions of "Part:BBa K2012009"

Revision as of 14:19, 15 October 2016

RBS(J61100) and CheZ with fused with a GS linker and GFP in C teminal

Bacteria movement related gene CheZ which fused with a GS linker and GFP in C teminal. We will use this part to charactise the expression of CheZ. These parts are present in plasmid pSB1A2, but there is also a constitutive promoter (J23100-derived) inserted into the XbaI site. So, for example, the EcoRI/PstI region of part J61100 reads: Biobrick 5' XbaI J23100 XbaI '''RBS Part''' Biobrick 3' gaattcgcggccgcttctagaGTTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCTtctaga'''GAAAGAGGGGACAA'''actagtagcggccgctgcag This feature in no way prevents the use of these parts in standard Biobrick assembly. Normal prefix insertion into EcoRI/XbaI will delete this promoter element. Suffix insertion into SpeI/PstI will retain this promoter, but it can of course be removed later by a prefix insertion. Note also that the base 5' to the SpeI site is allowed to float in these parts and is therefore rarely "T". The "G" downstream of the XbaI site obeys the standard. Because the database does not permit variation at this position, the predicted sequences of composite parts derived from these parts will be incorrect at this position.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1321

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 Bacteria movement related gene CheZ which fused with a GS linker and GFP in C teminal. We will use this part to charactise the expression of CheZ.
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+These parts are present in plasmid pSB1A2, but there is also a constitutive promoter (J23100-derived) inserted into the XbaI site.  So, for example, the EcoRI/PstI region of part J61100 reads:
+  Biobrick 5'    XbaI                    J23100              XbaI    '''RBS Part'''     Biobrick 3'
+  gaattcgcggccgcttctagaGTTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCTtctaga'''GAAAGAGGGGACAA'''actagtagcggccgctgcag
+This feature in no way prevents the use of these parts in standard Biobrick assembly.  Normal prefix insertion into EcoRI/XbaI will delete this promoter element.  Suffix insertion into SpeI/PstI will retain this promoter, but it can of course be removed later by a prefix insertion.
+Note also that the base 5' to the SpeI site is allowed to float in these parts and is therefore rarely
+"T".  The "G" downstream of the XbaI site obeys the standard.  Because the database does not permit variation at this position, the predicted sequences of composite parts derived from these parts will be incorrect at this position.
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